FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18908770
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG140150.09.25221794-95
TCGTGTA93750.09.09430494-95
CGTGTAG90950.09.00871294-95
CGTCGTG113750.08.97766494-95
GCGTCGT120500.08.94777694-95
ATCGGAA334350.08.88591894-95
GTCGTGT106650.08.41738694-95
GATCGGA359850.08.3882394-95
GAGCGTC175550.08.30641994-95
TCGGAAG323400.07.769479394-95
AGAGCGT188050.07.716389794-95
AGATCGG399450.07.61016194-95
CGGAAGA329850.07.595952594-95
AAGAGCG224000.07.43218194-95
TAGATCG123350.07.1622694-95
GTGTAGG123750.06.10278794-95
AAGATCG135850.06.101155394-95
GAGATCG149200.06.048686594-95
CAGATCG141000.05.878311692-93
GTAGGGA113950.05.27293694-95
Produced by FastQC (version 0.11.5)