FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22107536
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACA30300.020.3891941
ATCGTAC24600.018.8217616-17
GATCGTA25100.016.8386416-17
GATTAGG54550.016.53939
TCGTCGA27900.016.51460840-41
GTCGTAA26000.016.1733354-55
CGGTCTG57500.015.7857351
TACGATC22850.014.44816438-39
CGTCGAG30600.014.20374642-43
CGACAGT44100.014.0004083
TCGTACC31650.013.57890118-19
GTACGAT21900.013.231215538-39
GGATCGT35350.013.030416514-15
TCGTAAG32600.012.896317556-57
CGTAAGA33300.012.69655456-57
AGATTAG74250.012.6627218
CGTACCA38100.011.90332118-19
CTCGACG28200.011.53477512-13
ACGGGTT38150.011.39216328-29
TCGACGA26100.011.37144214-15
Produced by FastQC (version 0.11.5)