FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21343635
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG91450.09.9570294-95
AGCGTCG110050.09.81200794-95
GCGTCGT94700.09.56496494-95
GTCGTGT86650.09.51825394-95
ATCGGAA297400.09.35361894-95
GATCGGA322100.09.33198694-95
TCGTGTA72900.09.15546194-95
AGATCGG364750.09.05115994-95
TCGGAAG293250.09.01453394-95
GAGCGTC146150.08.82364894-95
TAGATCG111150.08.728494-95
AGAGCGT159850.08.64898394-95
CGGAAGA299550.08.35544794-95
AAGAGCG194550.07.976262694-95
CGTGTAG75050.07.87681694-95
AAGATCG133900.06.924983594-95
CAGATCG136100.06.760500494-95
GAGATCG150400.06.101865394-95
TCCGATC23656.730261E-116.02271183
TTACGCG4350.0090584216.006582748-49
Produced by FastQC (version 0.11.5)