FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21737583
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA377200.08.490602594-95
GATCGGA408300.08.43115194-95
TCGGAAG374900.08.36537994-95
AGATCGG458100.08.20387294-95
ACACGTC137800.08.1491194-95
CGTCTGA97050.08.12158594-95
TAGATCG135300.07.93119994-95
CGGAAGA374550.07.87245294-95
CACACGT171350.07.592673394-95
ACGTCTG123500.07.22801794-95
CACGTCT139250.07.041304694-95
GCACACG198200.07.01927494-95
AAGATCG148150.06.45805394-95
CAGATCG159200.06.218580294-95
GAGATCG197450.06.14415394-95
AGAGCAC328500.05.83948194-95
AGCACAC279400.05.701568694-95
AAGAGCA425000.05.418524794-95
GAAGAGC472550.05.395786394-95
GAGCACA335850.05.28048194-95
Produced by FastQC (version 0.11.5)