FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004719982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004719982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21113335
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC142700.09.45196394-95
CGTCTGA102300.08.86717894-95
ATCGGAA429450.08.85271494-95
TCGGAAG413400.08.75411394-95
GATCGGA463900.08.75325294-95
AGATCGG522200.08.53542394-95
CACACGT175500.08.34844794-95
ACGTCTG124100.08.30454194-95
CGGAAGA404550.08.25884994-95
GCACACG202450.07.98779794-95
CACGTCT145000.07.92638294-95
TAGATCG171050.07.4966794-95
CAGATCG174450.07.323336694-95
GAGATCG194300.07.08847694-95
AAGATCG163850.06.855077794-95
AGAGCAC343600.06.72445994-95
AGCACAC291200.06.40141594-95
GAGCACA346250.05.973461694-95
AAGAGCA458350.05.87507694-95
GAAGAGC497400.05.705054894-95
Produced by FastQC (version 0.11.5)