FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21033612
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG118250.09.30725394-95
CGTCGTG99250.09.07716894-95
GCGTCGT102850.09.01367794-95
GTCGTGT93450.08.92831594-95
ATCGGAA296300.08.61617294-95
AGATCGG359450.08.550794-95
GATCGGA316100.08.52014794-95
TCGTGTA80800.08.50213994-95
GAGCGTC150600.08.44443394-95
AGAGCGT169350.08.30956394-95
AAGAGCG199900.08.25255394-95
TCGGAAG293300.08.11266794-95
TAGATCG117450.08.03487594-95
CGTGTAG79400.07.78385194-95
CGGAAGA295400.07.749211394-95
AAGATCG129450.06.610619594-95
CAGATCG131450.06.473871794-95
GAGATCG150100.06.176260594-95
GAAGAGC397700.05.116345494-95
GTGTAGG121750.05.01771394-95
Produced by FastQC (version 0.11.5)