FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19981739
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT104050.09.72320394-95
CGTCGTG98550.09.68748994-95
AGCGTCG121450.09.56209894-95
TCGTGTA78450.09.53585194-95
CGTGTAG82000.09.03613394-95
ATCGGAA312150.09.00803894-95
GATCGGA337400.09.00258594-95
AGATCGG382550.08.88368894-95
TAGATCG117400.08.77937294-95
GTCGTGT91200.08.59331994-95
AAGAGCG207200.08.42440894-95
GAGCGTC155650.08.20871194-95
AGAGCGT171700.08.18829194-95
TCGGAAG301850.08.11165294-95
CGGAAGA307900.07.867419794-95
AAGATCG140050.07.03731294-95
CAGATCG134950.06.77531794-95
GAGATCG146800.05.856313794-95
GAAGAGC404750.05.433328694-95
GTGTAGG120850.05.345201594-95
Produced by FastQC (version 0.11.5)