FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21227932
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG125100.09.64655294-95
AGCGTCG154200.09.3050494-95
GCGTCGT132300.09.19339394-95
GTCGTGT115200.08.90834494-95
TCGTGTA102250.08.64261794-95
GAGCGTC191050.08.59204994-95
GATCGGA401350.08.33975894-95
ATCGGAA370850.08.28899194-95
AGATCGG449450.08.28234594-95
AAGAGCG252000.08.17305294-95
TCGGAAG361250.08.06210294-95
AGAGCGT213700.08.03710294-95
CGGAAGA365050.07.978179594-95
TAGATCG143750.07.93229994-95
CGTGTAG101150.07.70324494-95
AAGATCG151500.06.711148394-95
CAGATCG154750.06.07897394-95
GAGATCG169900.05.99831994-95
GTAGGGA131700.05.483444794-95
GTGTAGG138900.05.472848494-95
Produced by FastQC (version 0.11.5)