FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19974087
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA369650.09.28238994-95
AGATCGG420100.09.12723694-95
TAGATCG134100.09.01827294-95
ATCGGAA338900.08.96312694-95
CGTCTGA86850.08.57317794-95
TCGGAAG327000.08.41186394-95
CGGAAGA328100.08.11625294-95
ACACGTC114450.07.66598494-95
CACACGT146650.07.55121494-95
ACGTCTG106850.07.501085894-95
GCACACG171150.07.481674794-95
AAGATCG131600.07.04535494-95
CAGATCG149950.06.673412394-95
CACGTCT122450.06.448629494-95
GAGATCG165200.06.21526794-95
AGAGCAC285300.06.192078694-95
GAAGAGC419300.05.6666394-95
AAGAGCA391450.05.506422594-95
AGCACAC250500.05.3862594-95
GAGCACA289450.05.08744894-95
Produced by FastQC (version 0.11.5)