FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19974087
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG108550.09.582581594-95
GCGTCGT114850.09.40846394-95
AGCGTCG129850.09.29094894-95
GATCGGA362900.09.10287194-95
AGATCGG411700.08.95259894-95
AAGAGCG221200.08.77152694-95
ATCGGAA331300.08.76684494-95
CGTGTAG90450.08.74327194-95
GTCGTGT98900.08.69261694-95
TCGTGTA87650.08.61615494-95
TAGATCG132150.08.28460994-95
AGAGCGT180750.08.11985894-95
GAGCGTC162250.07.96255594-95
TCGGAAG319350.07.93479594-95
CGGAAGA325700.07.91863594-95
AAGATCG124800.07.11697294-95
CAGATCG152600.06.614128694-95
GTGTAGG124000.06.262738794-95
GAGATCG156250.06.125241894-95
GAAGAGC411750.05.513946594-95
Produced by FastQC (version 0.11.5)