FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21155433
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA409000.09.16697494-95
ATCGGAA377450.09.09759494-95
AGATCGG462100.08.88851794-95
TCGGAAG362250.08.84431894-95
CGGAAGA355750.08.32597394-95
TAGATCG149550.08.22997294-95
CACACGT152250.08.20863194-95
GCACACG172050.08.09097594-95
ACACGTC120350.08.03954594-95
ACGTCTG110800.07.51250594-95
CGTCTGA89450.07.39675594-95
AAGATCG155450.07.21585594-95
CACGTCT128000.07.207041794-95
CAGATCG157350.06.4957394-95
GAGATCG163850.06.455142594-95
AGAGCAC311700.06.215880494-95
AGCACAC268450.06.13958294-95
GAGCACA313000.05.85669494-95
GAAGAGC447300.05.741785594-95
AAGAGCA419750.05.395479794-95
Produced by FastQC (version 0.11.5)