FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21962361
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG144650.09.932878594-95
CGTCGTG118650.09.60752894-95
GCGTCGT123750.09.59539894-95
TCGTGTA96100.09.16829794-95
GAGCGTC185100.09.00676694-95
AAGAGCG244400.08.9980494-95
GTCGTGT107650.08.91262294-95
AGATCGG464300.08.85905594-95
AGAGCGT201950.08.79621794-95
GATCGGA411800.08.69667394-95
CGTGTAG93950.08.6450594-95
ATCGGAA378100.08.63642994-95
TCGGAAG364050.08.45438894-95
TAGATCG149000.08.27216694-95
CGGAAGA368550.08.14495894-95
AAGATCG155850.07.49715594-95
CAGATCG164550.07.201796594-95
GAGATCG176600.06.52212694-95
GTGTAGG134550.05.85993294-95
GAAGAGC461900.05.825324594-95
Produced by FastQC (version 0.11.5)