FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23075354
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA392200.09.19460694-95
GATCGGA421300.09.08920394-95
TCGGAAG384700.08.53459594-95
AGATCGG475000.08.27155894-95
TAGATCG140850.07.65212294-95
CGGAAGA384550.07.62424994-95
ACGTCTG127750.07.50763994-95
ACACGTC143000.07.42086294-95
CACGTCT147250.07.222800794-95
CACACGT174750.07.20015194-95
CGTCTGA103350.07.029004694-95
GCACACG207100.06.7976394-95
AAGATCG157800.06.544329694-95
CAGATCG167350.06.383659494-95
AGAGCAC343700.05.84350994-95
AGCACAC291750.05.761098494-95
GAGATCG199600.05.649577694-95
GAGCACA345600.05.619044394-95
AAGAGCA446100.05.279129594-95
GAAGAGC487450.05.24527894-95
Produced by FastQC (version 0.11.5)