FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23075354
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA103050.09.80129794-95
AGCGTCG158750.09.73065994-95
GCGTCGT140050.09.48574394-95
ATCGGAA388100.09.22809994-95
CGTCGTG132250.09.14670794-95
GATCGGA421000.09.01499594-95
GTCGTGT118650.08.79304894-95
CGTGTAG108750.08.65383494-95
GAGCGTC202850.08.59931294-95
TCGGAAG384200.08.45578294-95
AGAGCGT218400.08.44406894-95
AGATCGG473500.08.20616894-95
AAGAGCG262200.08.093966594-95
TAGATCG139250.07.79944494-95
CGGAAGA384150.07.65264494-95
AAGATCG162350.06.616506694-95
CAGATCG177550.06.050070394-95
GAGATCG188450.05.586633794-95
GTGTAGG144500.05.394468394-95
GAAGAGC502150.05.10658694-95
Produced by FastQC (version 0.11.5)