FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22330965
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC55350.010.55291751
ATCGGAA373050.09.24176994-95
GATCGGA404000.09.13324694-95
AGATCGG456700.08.78120694-95
TCGGAAG364250.08.7675594-95
CGGAAGA359800.08.35464794-95
TAGATCG144700.07.909227494-95
ACACGTC124150.07.688380794-95
CTTTACG25600.07.60064842
CGTCTGA94000.07.57790294-95
ACTTAAC64700.07.41313931
CACGTCT136750.07.25779194-95
ACGTCTG118800.07.195178594-95
ACCGTGC72200.07.169261
GCACACG184900.07.16560494-95
ACCGCAC52700.07.1187141
AAGATCG152800.06.961618494-95
CACACGT156450.06.84473394-95
ACCTAAC51400.06.8368121
ACTTTAT151200.06.78401851
Produced by FastQC (version 0.11.5)