FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21975852
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG114950.010.10308594-95
AGCGTCG143350.09.89078494-95
GCGTCGT120600.09.64945794-95
GTCGTGT104750.09.63582794-95
TCGTGTA91700.09.50497294-95
ATCGGAA371000.09.3589694-95
GATCGGA404950.09.26050994-95
AGATCGG456500.08.89109894-95
CGTGTAG91050.08.81639194-95
AAGAGCG240800.08.708894-95
GAGCGTC180400.08.70294-95
TAGATCG146850.08.58765694-95
AGAGCGT201900.08.53993294-95
TCGGAAG356450.08.32181394-95
CGGAAGA355350.08.24732294-95
AAGATCG147750.06.960084494-95
CAGATCG163300.06.631820794-95
GAGATCG173900.06.39146494-95
GTGTAGG128450.05.861101694-95
GAAGAGC454650.05.364723794-95
Produced by FastQC (version 0.11.5)