FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21933574
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG103700.010.15233194-95
GCGTCGT89500.09.46224394-95
GATCGGA278350.09.34009794-95
CGTCGTG85950.09.25409294-95
GTCGTGT81050.09.21013994-95
GAGCGTC137200.09.19312694-95
TCGTGTA73650.08.98217794-95
AGATCGG317250.08.92508394-95
ATCGGAA260650.08.91766794-95
AGAGCGT152400.08.74915494-95
TCGGAAG262050.08.70303694-95
AAGAGCG177550.08.36520194-95
TAGATCG101650.08.30591994-95
CGTGTAG76000.08.0609294-95
CGGAAGA270500.07.917303694-95
AAGATCG120300.06.52612694-95
CAGATCG125550.06.191722494-95
GAGATCG140450.05.91973294-95
CGCGATA6300.00359707815.494862684-85
GTGTAGG114950.05.21756994-95
Produced by FastQC (version 0.11.5)