FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20836334
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG129100.09.07130594-95
GTCGTGT101800.08.86716894-95
CGTCGTG112000.08.82316694-95
GATCGGA342750.08.70485194-95
ATCGGAA316300.08.6592394-95
TCGTGTA90450.08.48287594-95
GCGTCGT117100.08.47946694-95
AGATCGG385450.08.36914194-95
GAGCGTC165450.08.356132594-95
AGAGCGT183000.08.26870594-95
TCGGAAG310200.08.14030494-95
AAGAGCG215600.07.999024494-95
TAGATCG121950.07.986402594-95
CGGAAGA315100.07.546313394-95
CGTGTAG89400.07.3602394-95
CAGATCG138550.06.840935794-95
AAGATCG135650.05.883945594-95
GAGATCG151650.05.81139894-95
GTGTAGG125500.05.735193794-95