FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21486800
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA114100.09.15872494-95
CGTCGTG138700.08.83570494-95
CGTGTAG112100.08.62296794-95
GTCGTGT127800.08.62293394-95
AGCGTCG164550.08.60233494-95
GCGTCGT145450.08.5562994-95
ATCGGAA372500.07.91887194-95
GATCGGA401200.07.843734394-95
GAGCGTC203350.07.836935594-95
AAGAGCG260150.07.76002394-95
TAGATCG135350.07.72080194-95
AGATCGG443500.07.652556494-95
AGAGCGT222850.07.63076994-95
TCGGAAG362500.07.285589794-95
CGGAAGA369150.07.012801694-95
GTGTAGG147700.06.2229894-95
AAGATCG147700.06.046099794-95
CAGATCG163050.05.97215494-95
GAGATCG166950.05.605026794-95
GTAGGGA133450.05.42811794-95
Produced by FastQC (version 0.11.5)