FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22083113
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG142200.010.454679594-95
AGCGTCG175150.010.02006194-95
GTCGTGT130200.09.83136594-95
GCGTCGT149700.09.42324994-95
TCGTGTA111200.09.39688594-95
ATCGGAA445150.09.38949794-95
CGTGTAG109750.09.32628694-95
GATCGGA484700.08.86342494-95
TCGGAAG418300.08.72045994-95
TAGATCG176200.08.66644794-95
GAGCGTC215900.08.62381694-95
AGATCGG546000.08.56424594-95
AAGAGCG279250.08.56391394-95
CGGAAGA413250.08.51095394-95
AGAGCGT236650.07.8877394-95
AAGATCG172050.06.887821794-95
CAGATCG187200.06.66023494-95
GTGTAGG147650.06.385473394-95
GAGATCG201350.06.345511494-95
GAAGAGC498950.05.81158694-95