FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21990206
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA338700.08.798861594-95
ATCGGAA319250.08.62085794-95
AGATCGG382100.08.57008694-95
TAGATCG129400.08.31307894-95
TCGGAAG311050.07.832764694-95
ACACGTC114250.07.71108794-95
CGTCTGA88050.07.57835494-95
ACCGCGC41850.07.50044631
CGGAAGA316800.07.35329194-95
AAGATCG139150.06.77493694-95
CAGATCG130700.06.68605894-95
ACGTCTG110400.06.646413394-95
CACACGT148050.06.560135494-95
CACGTCT127900.06.516801494-95
GCACACG166900.06.50216294-95
AGAGCAC289150.06.052603294-95
ACTGCGC51050.05.86926031
GAGATCG149950.05.70103894-95
AGCACAC249450.05.226208794-95
GAAGAGC432500.05.221464694-95
Produced by FastQC (version 0.11.5)