FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20853640
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA413050.08.34473294-95
AGATCGG498300.08.29003494-95
GATCGGA444600.08.286859594-95
CACACGT176950.08.28289694-95
ACACGTC138500.08.23263994-95
TCGGAAG402650.08.22399194-95
GCACACG202300.08.19609894-95
CGTCTGA105800.08.12775394-95
ACGTCTG128850.08.11177494-95
CGGAAGA393550.07.90109694-95
TAGATCG161800.07.7664994-95
CACGTCT148000.07.52763894-95
AAGATCG169050.07.30694494-95
CAGATCG156650.06.611556594-95
GAGATCG181000.06.535794794-95
AGAGCAC340300.06.415066794-95
AGCACAC291600.05.987526494-95
GAGCACA343850.05.8514394-95
GAAGAGC485800.05.388547494-95
AAGAGCA449050.05.33757694-95
Produced by FastQC (version 0.11.5)