FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20853640
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG137600.010.11495494-95
GCGTCGT148050.09.86623594-95
AGCGTCG167800.09.66748594-95
TCGTGTA110800.09.28181494-95
CGTGTAG108550.09.1022494-95
GTCGTGT124250.08.92699494-95
GAGCGTC209700.08.56264894-95
ATCGGAA401550.08.36953994-95
AGAGCGT231050.08.25456894-95
AGATCGG487150.08.21039794-95
TAGATCG152300.08.20295994-95
GATCGGA433450.08.15358694-95
AAGAGCG267850.08.11361194-95
TCGGAAG392250.07.974575594-95
CGGAAGA383000.07.912917694-95
AAGATCG161850.06.67703294-95
GAGATCG176950.06.50992494-95
GTGTAGG142350.06.47379694-95
CAGATCG165800.06.403357594-95
GAAGAGC467000.05.365625494-95
Produced by FastQC (version 0.11.5)