FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19812135
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA87100.010.49777194-95
CGTCGTG108750.010.43886494-95
AGCGTCG133400.09.91639894-95
GTCGTGT98800.09.9036494-95
GCGTCGT113600.09.76322194-95
CGTGTAG86250.09.1969194-95
AGATCGG439050.09.15252694-95
GAGCGTC171550.09.12324394-95
ATCGGAA352600.08.90438194-95
GATCGGA384800.08.83817494-95
AAGAGCG222350.08.61967794-95
CGGAAGA336250.08.52510294-95
TAGATCG142650.08.474236594-95
AGAGCGT185850.08.35737394-95
TCGGAAG337150.08.22762194-95
AAGATCG142100.07.3371194-95
GAGATCG160300.06.75594294-95
CAGATCG151400.06.509937394-95
GAAGAGC419200.06.0563594-95
GGAAGAG589200.05.25213994-95
Produced by FastQC (version 0.11.5)