FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23289747
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG137250.09.60881394-95
TCGTGTA112200.09.55152794-95
GTCGTGT126150.09.26759394-95
GCGTCGT145600.09.09039994-95
ATCGGAA395650.08.94278294-95
AGCGTCG163700.08.85462794-95
GATCGGA425950.08.6246294-95
GAGCGTC204550.08.2712294-95
AGAGCGT227750.08.22161194-95
TCGGAAG389900.08.154494-95
CGTGTAG112450.08.09335194-95
AAGAGCG270200.07.879738394-95
AGATCGG478400.07.86779694-95
CGGAAGA386750.07.729286794-95
TAGATCG149150.07.63134994-95
CAGATCG171600.06.328314394-95
AAGATCG158950.06.129321694-95
GAGATCG195300.05.54819594-95
GTGTAGG149300.05.25222494-95
GAAGAGC498700.05.1126994-95
Produced by FastQC (version 0.11.5)