FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21927793
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG130750.09.84471194-95
AGCGTCG162900.09.52001294-95
TCGTGTA106850.09.42403194-95
GCGTCGT137900.09.26538394-95
GTCGTGT120650.09.21221294-95
CGTGTAG103900.08.82301794-95
GAGCGTC200150.08.80427794-95
GATCGGA437950.08.71980894-95
ATCGGAA404000.08.70600994-95
AGATCGG493800.08.58965694-95
AAGAGCG264100.08.54280794-95
AGAGCGT221200.08.53546994-95
TAGATCG157250.08.33669494-95
TCGGAAG390950.08.15830994-95
CGGAAGA388950.08.10867194-95
AAGATCG166900.07.18588894-95
GAGATCG182450.06.443275594-95
CAGATCG166900.06.3036694-95
GTGTAGG139100.05.770785394-95
GAAGAGC483200.05.327797494-95
Produced by FastQC (version 0.11.5)