FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20778535
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC132900.09.22323994-95
GATCGGA431050.08.90027994-95
ATCGGAA397500.08.87458594-95
AGATCGG482200.08.690207594-95
TCGGAAG385150.08.52387194-95
CACACGT167850.08.36420994-95
CGTCTGA98400.07.966683494-95
ACGTCTG124300.07.892919594-95
TAGATCG155050.07.844349494-95
CACGTCT140600.07.788867594-95
CGGAAGA381150.07.74700794-95
GCACACG191500.07.46769394-95
AAGATCG164750.07.12295694-95
CAGATCG152250.06.724789694-95
GAGATCG180800.06.30669194-95
AGAGCAC334000.06.201956794-95
GAAGAGC466100.05.6928994-95
AGCACAC282350.05.67903794-95
GAGCACA330700.05.52396494-95
AAGAGCA444400.05.495127794-95
Produced by FastQC (version 0.11.5)