FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20778535
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG129100.010.21110894-95
GTCGTGT116500.010.07180194-95
TCGTGTA102950.010.01312394-95
GCGTCGT136350.09.476541594-95
CGTGTAG100300.09.33042594-95
AGCGTCG154600.09.15678394-95
ATCGGAA390750.08.85051494-95
GATCGGA424200.08.813333594-95
AGATCGG475650.08.60906394-95
AAGAGCG255650.08.5662694-95
GAGCGTC195150.08.55643694-95
AGAGCGT215300.08.32931194-95
TCGGAAG377450.08.256206594-95
TAGATCG145250.08.07824494-95
CGGAAGA369650.07.83283894-95
AAGATCG160750.06.959468494-95
CAGATCG163450.06.55386994-95
GTGTAGG134050.06.18393394-95
GAGATCG176000.06.005559494-95
GAAGAGC455000.05.65879694-95
Produced by FastQC (version 0.11.5)