FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20673432
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT96250.09.72502294-95
CGTCGTG89100.09.46554894-95
AGCGTCG108700.09.46353794-95
GATCGGA291500.08.86717994-95
TCGTGTA75950.08.78969294-95
ATCGGAA269450.08.73756994-95
CGTGTAG76950.08.67546794-95
GAGCGTC141700.08.44998394-95
TAGATCG106600.08.446506594-95
GTCGTGT86300.08.39622794-95
AGATCGG326400.08.34849994-95
AGAGCGT159250.08.26466894-95
TCGGAAG265450.08.01005494-95
AAGAGCG185050.07.677276694-95
CGGAAGA271600.07.443804394-95
AAGATCG123000.06.238677594-95
CAGATCG122100.05.60364292-93
GTGTAGG114850.05.585060694-95
GAGATCG132800.05.45628394-95