FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21496358
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT87750.010.20401694-95
AGCGTCG101600.09.82600694-95
CGTCGTG83900.09.53756394-95
ATCGGAA247800.09.42808594-95
GATCGGA267400.09.37209194-95
TCGTGTA68700.09.10098794-95
AGATCGG301900.09.05107994-95
GAGCGTC131950.08.77489894-95
TCGGAAG252100.08.63448694-95
GTCGTGT79200.08.609138594-95
AAGAGCG172100.08.32747494-95
AGAGCGT149000.08.30611294-95
CGTGTAG72900.08.08252394-95
TAGATCG97100.07.79053194-95
CGGAAGA260350.07.2243594-95
CAGATCG120700.06.73625994-95
AAGATCG111100.06.484596394-95
GAGATCG133950.05.666544494-95
GTGTAGG112350.05.198663794-95
CGCGTAT6600.0092851545.06802194-95
Produced by FastQC (version 0.11.5)