FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21000665
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG139200.010.28784494-95
TCGTGTA89400.09.64307194-95
CGTCGTG112850.09.61747194-95
ATCGGAA357050.09.38521594-95
GCGTCGT119600.09.31296494-95
GATCGGA392250.09.28165694-95
GTCGTGT103600.09.14659594-95
GAGCGTC176600.08.99666894-95
CGTGTAG89850.08.82825194-95
AGATCGG440800.08.62034694-95
AGAGCGT194000.08.43458794-95
AAGAGCG231050.08.40801794-95
TCGGAAG341450.08.15862994-95
CGGAAGA348250.07.91748794-95
TAGATCG140250.07.75548394-95
GAGATCG169250.06.76339394-95
CAGATCG161100.06.70752394-95
AAGATCG141750.06.215801294-95
GTGTAGG124850.05.915863594-95
GAAGAGC449100.05.383330394-95
Produced by FastQC (version 0.11.5)