FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20870946
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG159050.011.16997294-95
GTCGTGT142400.011.14167994-95
TCGTGTA127150.010.90889294-95
CGTGTAG119350.010.70641494-95
AGCGTCG202800.010.55215294-95
GCGTCGT174750.010.51981294-95
GAGCGTC247550.09.87271694-95
AGAGCGT279200.09.65527494-95
AAGAGCG329900.09.43133494-95
GATCGGA575250.08.97195894-95
ATCGGAA530300.08.85908494-95
TCGGAAG502650.08.77466194-95
AGATCGG645450.08.70267494-95
CGGAAGA489150.08.52156394-95
TAGATCG200400.08.20148694-95
GTGTAGG149600.07.557175694-95
AAGATCG204150.07.376053394-95
CAGATCG206100.07.225596494-95
GAGATCG221100.07.03617694-95
GAAGAGC545350.06.78105894-95
Produced by FastQC (version 0.11.5)