FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22660643
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA101350.09.80099794-95
GTCGTGT117500.09.60668494-95
AGCGTCG152100.09.18683394-95
CGTCGTG128300.08.81653694-95
ATCGGAA367900.08.7653294-95
GCGTCGT136550.08.66673294-95
GATCGGA397700.08.50887594-95
AGAGCGT212550.08.251121594-95
AGATCGG446150.08.20271394-95
GAGCGTC193450.08.19360294-95
TCGGAAG366800.08.17612894-95
CGTGTAG100050.07.95692894-95
AAGAGCG250450.07.771065794-95
TAGATCG130600.07.678687694-95
CGGAAGA368050.07.457493394-95
CAGATCG167650.06.64793994-95
AAGATCG149700.05.984629694-95
GAGATCG185250.05.70846794-95
GTGTAGG138300.05.326687394-95
GAAGAGC476100.05.061246494-95
Produced by FastQC (version 0.11.5)