FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22780450
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA320150.09.08758794-95
AGATCGG363800.08.75451594-95
ATCGGAA296800.08.7142594-95
TCGGAAG298400.08.11834294-95
CGGAAGA305050.07.67665394-95
TAGATCG115050.07.555452394-95
ACACGTC108500.07.464327394-95
CGTCTGA88450.07.223047794-95
GCACACG163650.06.806484794-95
ACGTCTG104500.06.795491794-95
CACACGT140450.06.7132894-95
AAGATCG136400.06.59919394-95
CACGTCT121550.06.35030294-95
CAGATCG131100.06.231918394-95
GAGATCG155000.05.73067794-95
AGAGCAC292850.05.63644994-95
Produced by FastQC (version 0.11.5)