FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21329664
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG122750.010.19625694-95
AGCGTCG150950.09.88048294-95
TCGTGTA97800.09.81056794-95
GCGTCGT130100.09.56545494-95
GTCGTGT110800.09.53831994-95
GAGCGTC192500.09.51207494-95
GATCGGA446350.09.332664594-95
ATCGGAA407000.09.29550594-95
AGAGCGT217200.09.29417294-95
AAGAGCG253600.09.26186994-95
AGATCGG499900.08.86502794-95
CGTGTAG96500.08.83524894-95
CGGAAGA385150.08.42310894-95
TCGGAAG381200.08.30479394-95
TAGATCG161600.08.27405994-95
AAGATCG165050.07.093866394-95
CAGATCG173100.06.51700694-95
GAGATCG180650.06.44183694-95
TCGGAAA52900.06.150585794-95
GTGTAGG136250.05.961302894-95
Produced by FastQC (version 0.11.5)