FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19894490
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG123050.09.78479194-95
GTCGTGT114300.09.51578194-95
TCGTGTA100500.08.83752794-95
AGCGTCG145800.08.58377594-95
GCGTCGT130950.08.57789894-95
GAGCGTC182800.08.47025994-95
GATCGGA376950.08.36641894-95
ATCGGAA348500.08.32709894-95
CGTGTAG98900.07.97199594-95
AAGAGCG239250.07.911003694-95
AGATCGG424450.07.900114594-95
AGAGCGT199650.07.898124794-95
TAGATCG134100.07.50865894-95
CGGAAGA343700.07.448425394-95
TCGGAAG338300.07.1952794-95
AAGATCG135400.06.38422194-95
CAGATCG151900.05.940883694-95
GTGTAGG129400.05.92779994-95
GAGATCG158650.05.613276594-95
TGTAGGG145550.05.13953294-95
Produced by FastQC (version 0.11.5)