FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22662179
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA408900.09.39364494-95
GATCGGA441350.09.26741294-95
TCGGAAG391050.09.02158894-95
AGATCGG499950.08.95467294-95
CGGAAGA383850.08.59127594-95
CGTCTGA97950.08.52594694-95
TAGATCG165200.08.44445594-95
ACACGTC132800.08.39661394-95
ACGTCTG119700.08.02722294-95
CACACGT166300.07.87501194-95
GCACACG191600.07.65239494-95
CACGTCT136150.07.440713494-95
AAGATCG177350.06.99640394-95
CAGATCG163350.06.840788494-95
GAGATCG183200.06.539887494-95
AGAGCAC331800.06.22796594-95
AGCACAC285950.05.940558494-95
GAGCACA333950.05.470331794-95
GAAGAGC486850.05.433554694-95
AAGAGCA446400.05.33066294-95
Produced by FastQC (version 0.11.5)