FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21928983
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT123750.09.61992694-95
TCGTGTA94150.09.38862294-95
AGCGTCG141050.09.26549994-95
CGTCGTG114350.08.935348594-95
GTCGTGT106650.08.86750594-95
AGAGCGT199500.08.50422194-95
ATCGGAA344200.08.48438394-95
GAGCGTC183300.08.43913294-95
TCGGAAG344300.08.32318494-95
CGTGTAG96600.08.24037494-95
GATCGGA370550.08.22733394-95
AGATCGG419900.08.21674894-95
TAGATCG127400.07.94546694-95
AAGAGCG236550.07.644356794-95
CGGAAGA347400.07.55124494-95
AAGATCG137350.06.41836694-95
CAGATCG163350.06.342296694-95
GAGATCG174700.05.807834694-95
GTGTAGG136050.05.519093594-95
GAAGAGC452450.05.20979194-95
Produced by FastQC (version 0.11.5)