FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18628727
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG92550.010.85460594-95
AGCGTCG114200.010.48128594-95
GCGTCGT98950.010.46455694-95
GTCGTGT84300.010.00117194-95
TCGTGTA72050.09.46014994-95
ATCGGAA295350.08.95773794-95
GAGCGTC148100.08.70752794-95
GATCGGA317450.08.55856294-95
AAGAGCG192150.08.45406994-95
TAGATCG114600.08.20655294-95
AGATCGG355600.08.13458994-95
CGTGTAG71550.08.13217294-95
TCGGAAG284400.08.10848594-95
AGAGCGT165400.07.897275494-95
CGGAAGA288950.07.88217394-95
AAGATCG122100.06.65212794-95
CAGATCG123600.06.033388692-93
GAAGAGC374900.05.669668794-95
GAGATCG138050.05.539487494-95
GTGTAGG107000.05.238154494-95
Produced by FastQC (version 0.11.5)