FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20470999
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC39050.013.511311
GATCGGA382500.09.03385194-95
ATCGGAA358250.08.92941194-95
TCGGAAG342350.08.62961594-95
CGGAAGA340200.08.52359494-95
ACACGTC121650.08.31646694-95
AGATCGG430850.08.24055594-95
TAGATCG144850.07.91899494-95
ACCGCAC40400.07.76529741
CGTCTGA94500.07.539296694-95
ACTGCGC43750.07.49663931
CACACGT150950.07.34725594-95
GCACACG169850.07.20083894-95
AAGATCG150100.07.16735494-95
ACGTCTG114750.07.078068794-95
ACCGTGC54550.06.88379431
CACGTCT126750.06.7826994-95
AGAGCAC297800.06.37979494-95
GAGATCG160100.06.333997794-95
CCGCGCC88500.06.11850262
Produced by FastQC (version 0.11.5)