FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20470999
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG137500.09.38002494-95
CGTCGTG113600.09.32531294-95
GTCGTGT106600.09.29149694-95
GAGCGTC171600.09.107841594-95
TCGTGTA96700.09.01459994-95
GCGTCGT119350.08.95564694-95
GATCGGA379400.08.89618194-95
AAGAGCG223050.08.83858494-95
ATCGGAA351250.08.79767294-95
AGAGCGT190450.08.79255294-95
TCGGAAG333650.08.42171394-95
CGGAAGA333200.08.38318794-95
CGTGTAG94700.08.22679594-95
AGATCGG423300.08.19801694-95
TAGATCG137850.08.13283794-95
CAGATCG152950.07.050390794-95
AAGATCG138650.05.96164694-95
GTGTAGG130850.05.86321294-95
GAGATCG159600.05.744619494-95
GAAGAGC413600.05.513120794-95
Produced by FastQC (version 0.11.5)