FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23459325
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACA31000.012.1081641
TACGATC21450.09.294338-39
CGACAGT43700.09.01538853
ATCGGAA400700.08.94880394-95
GATCGGA429800.08.77911194-95
AGATCGG486850.08.70574994-95
TCGGAAG391750.08.45660994-95
GTACGAT21050.08.45617438-39
TCGACGA23750.08.39335314-15
CTCGACG25050.08.33671212-13
TAGATCG150550.08.24405294-95
GCACACG199900.07.953931394-95
CGGAAGA383900.07.856827794-95
GGTACGA27800.07.7695736-37
CACACGT170250.07.58283594-95
ACACGTC132700.07.51100894-95
CAGATCG173150.07.40101294-95
AAGATCG164150.06.88154494-95
ACGTCTG126850.06.80974494-95
AACCCGT30800.06.704352426-27
Produced by FastQC (version 0.11.5)