FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23459325
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC19900.010.38342816-17
AGCGTCG158000.010.20587494-95
GCGTCGT132950.09.84239594-95
CGTCGTG124600.09.43462694-95
GTCGTAA19200.09.15523254-55
GATCGTA21400.09.10069716-17
ATCGGAA389150.08.99534894-95
TCGTCGA22450.08.78068540-41
TAGATCG150200.08.7594694-95
GATCGGA420500.08.72004794-95
AGATCGG472950.08.52628894-95
GAGCGTC196100.08.47730794-95
AAGAGCG256400.08.47501894-95
TCGGAAG374150.08.13094294-95
TCGTGTA102850.08.01240294-95
GTCGTGT114700.07.929994694-95
AGAGCGT212400.07.860285394-95
CGGAAGA377250.07.793434694-95
GATTAGG53100.07.69256939
CGGTCTG53150.07.68533371
Produced by FastQC (version 0.11.5)