FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21067196
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA447000.09.16370894-95
GATCGGA484600.09.10441394-95
CACACGT176400.08.85761794-95
TCGGAAG426300.08.70072394-95
ACACGTC143400.08.66048494-95
GCACACG203650.08.58189294-95
CGGAAGA412750.08.49734194-95
AGATCGG540950.08.432569594-95
TAGATCG177650.08.31409294-95
CGTCTGA106150.08.16511994-95
ACGTCTG127600.07.55553194-95
CACGTCT147100.07.28037194-95
CAGATCG175450.06.929569294-95
AAGATCG175400.06.80970194-95
AGAGCAC349300.06.77096294-95
AGCACAC296850.06.207465694-95
GAGATCG192600.06.004306392-93
GAGCACA346950.05.98867694-95
AAGAGCA459150.05.797498794-95
GAAGAGC500450.05.627475794-95
Produced by FastQC (version 0.11.5)