FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21067196
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG140100.010.83529594-95
GCGTCGT152850.010.83291594-95
AGCGTCG174450.09.791294-95
GTCGTGT127250.09.63318594-95
TCGTGTA111500.09.20421894-95
AGAGCGT243600.09.10851694-95
GATCGGA480800.09.06670994-95
ATCGGAA444200.09.06502894-95
GAGCGTC214050.09.00085394-95
AAGAGCG285500.08.80354494-95
TCGGAAG428800.08.55956694-95
AGATCGG537150.08.42072394-95
CGGAAGA416900.08.31383594-95
CGTGTAG110250.08.29583694-95
TAGATCG165850.08.1789794-95
AAGATCG173150.06.942342394-95
GAGATCG193800.06.66842394-95
GTGTAGG142100.06.369552694-95
CAGATCG178900.06.227884394-95
GTAGGGA134550.05.755882394-95