FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20533849
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC44600.013.1077221
GATCGGA256350.09.84892694-95
AGATCGG292800.09.45837394-95
ATCGGAA238050.09.19923494-95
TAGATCG102200.09.1333694-95
ACCTCGC48100.08.8931251
ACTTAAC59300.08.8164741
ACTGCGC52500.08.419391
GCTTTAT117950.08.3412161
TCGGAAG235450.08.16091394-95
ACCGCAC45300.07.7640881
ACCGTGC62950.07.70125341
GCTTTAC86250.07.6597161
ACTGCAC190600.07.655481
ACTTTAC95300.07.38117931
ACTTCAC141850.07.20387361
ACTTTAT135300.07.16620731
CGGAAGA245050.07.12395392-93
ACCTAAC43650.07.0776011
CGTCTGA66600.07.025559494-95
Produced by FastQC (version 0.11.5)