FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20533849
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG87250.010.80605594-95
GATCGGA252000.09.80110294-95
AGATCGG289650.09.36342394-95
ATCGGAA232250.09.1314194-95
GCGTCGT75250.09.05769194-95
TAGATCG96850.08.80312694-95
AAGAGCG163350.08.66501394-95
GAGCGTC115750.08.658318594-95
CGTCGTG71050.08.42322794-95
TCGTGTA59500.08.1025494-95
AGAGCGT127300.08.09661494-95
GTCGTGT68050.08.09658694-95
TCGGAAG232950.07.992735494-95
ACCGCGC50750.07.9540291
CGTGTAG67450.07.253162494-95
ACGCGTA6600.00431138747.1959642
GCTTAAC43100.07.16210031
CGGAAGA240550.06.981186492-93
CAGATCG120300.06.85024294-95
ACTTAAC62650.06.6706151
Produced by FastQC (version 0.11.5)