FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20409433
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC144150.08.73022894-95
ATCGGAA420200.08.64569294-95
TCGGAAG404200.08.52384494-95
GATCGGA451100.08.44298394-95
CACGTCT143950.08.39596394-95
CGGAAGA395100.08.227365594-95
ACGTCTG124550.08.19764594-95
AGATCGG501800.08.17668894-95
CGTCTGA103300.08.04511294-95
CACACGT177050.07.993090694-95
TAGATCG160000.07.687306494-95
GCACACG200250.07.56506394-95
AAGATCG169250.07.3232994-95
AGAGCAC331950.06.65949694-95
CAGATCG160650.06.429436794-95
GAGATCG178750.06.01750694-95
AAGAGCA443200.05.887925694-95
GAAGAGC474950.05.864277494-95
AGCACAC287650.05.76176394-95
GAGCACA332400.05.67898294-95
Produced by FastQC (version 0.11.5)