FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21757975
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG158400.09.67687994-95
TCGTGTA104500.09.27843494-95
GCGTCGT135000.09.1185794-95
GTCGTGT117600.08.97235494-95
CGTCGTG128000.08.874598594-95
GAGCGTC194350.08.85288494-95
GATCGGA410400.08.69168894-95
ATCGGAA380050.08.62917294-95
AAGAGCG254000.08.53280694-95
AGAGCGT214400.08.51269794-95
AGATCGG463550.08.37182994-95
CGTGTAG104450.08.2817894-95
TAGATCG137600.08.18635794-95
TCGGAAG372100.07.77890494-95
CGGAAGA377950.07.388126494-95
AAGATCG156100.06.7137794-95
CAGATCG168650.06.524172394-95
GAGATCG183700.05.83442694-95
GTGTAGG142200.05.74896594-95
GAAGAGC475250.05.275469394-95
Produced by FastQC (version 0.11.5)