FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004720958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004720958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21277810
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA361650.08.85471794-95
ATCGGAA337500.08.49584894-95
AGATCGG405250.08.34757394-95
ACACGTC115200.08.31047494-95
TCGGAAG328750.08.20891794-95
CGGAAGA325250.07.975879794-95
TAGATCG134650.07.921605694-95
CGTCTGA87750.07.58026194-95
AAGATCG143950.07.21178994-95
CACACGT148550.07.148388494-95
CACGTCT119150.07.07795194-95
GCACACG167500.07.03461194-95
ACGTCTG106050.06.809828394-95
GAGATCG155350.06.208519594-95
CAGATCG128000.06.106012392-93
AGAGCAC298600.06.038446494-95
GAGCACA297600.05.64364694-95
AGCACAC258000.05.6075294-95
GAAGAGC433550.05.457495794-95
AAGAGCA411850.05.012495594-95
Produced by FastQC (version 0.11.5)